		Technical Data
K0199-17K	KRT14, ID (Keratin, Type I Cytoskeletal 14, Cytokeratin-14, CK-14, Keratin-14, K14)

Description:
Keratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. This protein, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. Keratin 14 expresses in most cells of squamous cell carcinomas, in spinous and suprabasal cells around the branching papillary region of papillomas, and weakly in a few proliferative cells of hyperplastic tissue.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml
Immunohistochemistry: 1.3ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.1mg/ml

Immunogen:

Synthetic peptide corresponding to the internal sequence of human keratin 14.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH7.4, 0.05% sodium azide.

Specificity:

Recognizes human keratin 14. Species Crossreactivity: mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Rosenberg, M. et al. (1988) Mol. Cell Biol. 8:722-736. 2. Bonifas, J.M. et al. (1991) Science 254: 1202-1205. 3. Van der Velden, L.A. et al. (1993) Head & Neck 15:133-146. 4. Chan, Y. et al. (1994) Genes Dev. 8:2574-2587. 5. Habib D. E. et al. Carginogenesis (2008) 29: 2377-84.