Technical Data
Media
Keratinocyte Basal Medium MCDB 153 w/o Methionine, Cysteine, Calcium (Powder)
K0200-01
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Ammonium Metavandate0.000585
Ammonium Molybdate•4H2O0.0012359
Calcium Chloride•2H2OAbsent
Cupric Sulfate•5H2O0.0027467
Ferric Nitrate•9H2O1.39
Magnesium Chloride57.13
Manganese Sulfate•5H2O0.00241
Nickel Chloride•6H2O0.00011885
Potassium Chloride111.83
Sodium Chloride7592
Sodium Metasilicate•9H2O0.1421
Sodium Phosphate Monobasic284.2
Sodium Selenite•5H2O0.00789
Stannous Chloride•2H2O0.0001128
Zinc Sulfate•7H2O0.14375
Amino Acids:
L-Alanine8.9
L-Arginine174.2
L-Asparagine13.21
L-Aspartic Acid3.993
L-CysteineAbsent
L-Glutamic Acid14.71
L-Glutamine876.0
Glycine7.5
L-Histidine12.416
L-Isoleucine1.97
L-Leucine65.6
L-Lysine14.62
L-MethionineAbsent
L-Phenylalanine4.956
L-Proline34.53
L-Serine63.03
L-Threonine11.91
L-Tryptophan3.063
L-Tyrosine2.718
L-Valine35.16
Vitamins:
Biotin0.012658
Choline Chloride13.96
Vitamin B120.40662
Folic Acid0.79452
Myo-Inositol18.02
Nicotinamide0.03663
D-Pantothenic Acid•Na0.2383
Pyridoxine•HCl0.02056
Riboflavin0.03764
Thiamine•HCl0.3373
Other:
Adenine24.318
Thymidine0.7266
D-Glucose1080.2
HEPES Free Acid6600.0
Lipoic Acid0.2063
Phenol Red, Sodium1.242
Putrescine•2HCl0.1611
Sodium Acetate303.4
Sodium Pyruvate55.0
Total:17.49g/L
MCDB media were designed for the low-protein or serum-free growth of specific cell types using hormones, growth factors, trace elements or low levels of dialyzed fetal bovine serum protein (FBSP). Each MCDB medium was formulated (qualitatively and quantitatively) to provide a defined and optimally balanced nutritional environment that selectively promoted growth of a specific cell type. MCDB 105 and 110 are modifications of MCDB 104 medium, optimized for long-term survival and rapid clonal growth of human diploid fibroblast-like cells (WI-38, MRC-5, IMR-90) and of low-passage human foreskin fibroblasts using FBSP or hormone and growth factor supplements. MCDB 151, 153, 201 and 302 are modifications of Ham's nutrient mixture F-12, designed for the growth of human keratinocytes, clonal growth of chicken embryo fibroblasts and chinese hamster ovary (CHO) cells using low levels of FBSP, extensive trace elements or no serum protein. The selection of a nutrient medium is strongly influenced by 1. type of cell; 2. type of culture (monolayer, suspension, clonal); and 3. degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line. MCDB 153 is a basal medium for the growth of normal keratinocytes and squamous epithelial cells after growth factor supplementation. Calcium content is low. Supplement with growth factors or fetal calf serum. K0200-01 is a basal medium lacking methionine and cysteine.


Directions per Liter: Dissolve 17.49g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 1.2g/l sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: White to pale orange, homogeneous, free flowing powder

Solubility: Pale beige to yellow, clear, complete

pH: As Reported

Endotoxin: 1EU/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Boyce, S.T.,Ham, R.G.: Calcium-Regulated Differentiation of Normal Human Epidermal Keratinocytes in Chemically Defined Clonal Culture and Serum-Free Serial Culture. J. Invest. Dermatol. 81: 33-40 (1983). 2. McKeehan, W.L., Ham, R.G.: Stimulation of Clonal Growth of Normal Fibroblasts with Substrata Coated with Basic Polymers. J. Cell Biol. 71: 727-734 (1976). 3. Hamilton, W.G., Ham, R.G.: Clonal Growth of Chinese Hamster Ovary Cell Lines in Protein-Free Media. In Vitro 13(9): 537-547 (1977).