Technical Data
Keratinocyte Growth Factor, Recombinant, Human (KGF, Fibroblast Growth Factor 7, FGF-7)
Molecular Biology Storage: -20CShipping: RT
KGF is a potent specific mitogen for many epithelial cells but not for fibroblasts and endothelial cells. KGF is thought to play an important role in the paracrine growth control of normal epithelial cells. KGF stimulates the proliferation of primary and secondary human keratinocytes to the same extent as EGF.

Recombinant Human KGF-1 (FGF-7) is a single, non-glycosylated, polypeptide chain containing 164 amino acids and having a molecular mass of 18995 Dalton.

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Cys-Asn-Asp-Met.

Biological Activity:
KGF is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent stimulation of KGF-responsive BaF3 indicator cells (measured by 3H-thymidine uptake) is < 10ng/ml corresponding to a specific activity of 10e5 Units/mg. Recombinant human KGF biological activity was measured by the KGF's mitogenic activity on BaF3 cells expressing the KGF receptor.

0.1ng/ug (IEU/ug) of KGF.

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 0.9 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of KGF as a Reference Standard.

Reconstitute in sterile ddH2O to a concentration 100ug/ml, which can then be further diluted to other aqueous solutions.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Note: For long term storage it is recommended to add a carrier protein (0.1% BSA or HSA).
Source: Recombinant, Human (E. coli)
Purity: 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: < 0.1ng/ug (IEU/ug).
Concentration: ~0.1mg/ml
Form: Supplied as a lyophilized powder. No additives. Reconstitute in sterile ddH2O to a concentration 100ug/ml, which can then be further diluted to other aqueous solutions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Washizawa, N., et al., JPEN J. Parenter. Enteral. Nutr. 28(6): 399-409 (2004). 2. Drugs R. D. 5(6): 351-354 (2004). 3. Orskov, C., et al., Regul. Pept. 124(1-3): 105-112 (2005). 4. Masui, F., et al., Cell Tissue Res. 318(3): 591-598 (2004). 5. Marti, G., et al., Gene Ther. 11(24): 1780-1785 (2004).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.