Technical Data
K0201-01A
Keratinocyte Growth Factor, Recombinant Human (KGF, Fibroblast Growth Factor 7, FGF-7)
10ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
KGF is a potent specific mitogen for many epithelial cells but not for fibroblasts and endothelial cells. KGF is thought to play an important role in the paracrine growth control of normal epithelial cells. KGF stimulates the proliferation of primary and secondary human keratinocytes to the same extent as EGF.

Recombinant human KGF is a single, non-glycosylated, polypeptide chain containing 164aa and having a molecular weight of 19kD. The sequence of the first five N-terminal aa was determined to be Met-Cys-Asn-Asp-Met. Contains less than 1% dimers and aggregates.

Activity:
The ED50 as determined by the dose-dependent stimulation of KGF-responsive BaF3 indicator cells (measured by 3H-thymidine uptake) is <10ng/ml, corresponding to a specific activity of 10Units/mg.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~19kD
Source: E. coli
Purity: 98% (HPLC, FPLC, SDS-PAGE)
Form: Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile ddH2O to a concentration 100ug/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.