Technical Data
K0201-15E
Keratinocyte Growth Factor, Recombinant, Human (KGF, Fibroblast Growth Factor 7, FGF7, FGF-7, Heparin-binding Growth Factor 7, HBGF-7)
2ug
Molecular Biology Storage: -20CShipping: Blue Ice
Source:
Recombinant corresponding to 164aa of human Keratinocyte Growth Factor expressed in E.coli is a single, non-glycosylated, polypeptide chain.

Molecular Weight:
~19kD

Biological Activity:
The ED50, calculated by the dose-dependent stimulation of KGF-responsive BaF3 indicator cells (measured by 3H-thymidine uptake).

Dimers and Aggregates:
1% (SDS-PAGE)

Protein Content:
Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 0.9 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of KGF as a Reference Standard.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~19kD
Purity: ~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
Concentration: ~1mg/ml
Form: Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Keratinocyte Growth Factor (KGF) Is Required For Post-Natal Thymic Regeneration. Blood 2005 Nov 22. 2. KGF induces lipogenic genes through a PI3K and JNK/SREBP-1 pathway in H292 cells. J Lipid Res 2005 Dec;46(12):2624-35. 3. KGF prevents oxygen-mediated damage in ARPE-19 cells. Invest Ophthalmol Vis Sci 2005 Sep;46(9):3435-42. 4. Stimulation of human hair growth by the recombinant human keratinocyte growth factor-2 (KGF-2). Biotechnol Lett 2005 Jun;27(11):749-52. 5. KGF-induced motility of breast cancer cells is dependent on Grb2 and Erk1,2. Clin Exp Metastasis 2004;21(5):437-43. 6. Comparative effects of glucagon-like peptide-2 (GLP-2), growth hormone (GH), and keratinocyte growth factor (KGF) on markers of gut adaptation after massive small bowel resection in rats. JPEN J Parenter Enteral Nutr 2004 Nov-Dec;28(6):399-409.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.