Technical Data
Ki-67 (Antigen Identified by Monoclonal Antibody Ki-67, KIA, MKI67, Proliferation Related Ki-67 Antigen)
Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumors is strong. Ki67 is routinely used as a marker of cell cycling and proliferation. This specificity of Ki-S5 antibody for proliferating cells might make it a useful tool for determination of the proliferative fraction in tumors such as Non-Hodgkin's lymphoma, mammary carcinomas, ovarian tumors and prostate cancer.

Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500. Detected cell-cycle-associated protein of 345kD and 395kD on 10ug of A431 lysates.
Immunocytochemistry: 5-10ug/ml
Immunohistochemistry: 5-10ug/ml
Optimal dilution determined by the researcher.

Recommended Secondary Antibodies:
I1904-02C: IgG, H&L (HRP) Pab Dk x Mo
I1904-03C: IgG, H&L (X-Adsorbed) (HRP) Pab Dk x Mo

Positive Control:
Tonsil tissue, A431 Cell Lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG12Q476Affinity Purified
100ug-20CBlue IceHumanMouse
Nuclear protein preparation from the human cell line U937.50.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide.
Recognizes a cell-cycle-associated protein of 345kD and 395kD identical with the human Ki-67 antigen. Binds to a formalin-resistant epitope of the Ki-67 antigen. Reactivity is confined to the nuclei of proliferating cells and no crossreactivity with cytoplasmic antigens of epithelial occurs even after antigen retrieval. Immunohistochemistry labeling of fresh and frozen samples showed identical results with Ki-67 and Ki-S5.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kreipe, H., et al., Am. J. Pathol. 142: 1689 (1993). 2. Mauri, F.A., et al., Applied Immunohistochemistry 2: 171 (1984). 3. Ballin, K., et al., Pathology Research and Practice 190: 269 (1994).