		Technical Data
K1700-10E	Ki-67 (Antigen Identified by Monoclonal Antibody Ki-67, KIA, MKI67, Proliferation Related Ki-67 Antigen)

Description:
The Ki-67 antigen is preferentially expressed during late G1, S, G2 and M phase of the cell cycle. Resting, non-cycling cells (G0 phase) lack the Ki-67 antigen. Constantly proliferating cells (e.g. cell lines) react positively during the entire cell cycle. This specificity for proliferating cells might make it a useful tool for determination of the proliferative fraction in tumors such as Non-Hodgkin’s lymphoma, mammary carcinomas, ovarian tumors and prostate cancer.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Immunohistochemistry: frozen and paraffin sections. Paraffin sections require boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
500ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.4mg/ml

Immunogen:

Synthetic peptide of Human Ki67. (Note: the amino acid sequence is proprietary). Cellular Localization: Nuclear

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide.

Specificity:

Recognizes human Ki67. Species Crossreactivity: rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Wharton SB et al. Expression of Ki67, PCNA and the chromosome replication licensing protein Mcm2 in glial cells of the ageing human hippocampus increases with the burden of Alzheimer-type pathology. Neurosci Lett 383:33-8 (2005). Dudderidge TJ et al. Mcm2, Geminin, and KI67 define proliferative state and are prognostic markers in renal cell carcinoma. Clin Cancer Res 11:2510-7 (2005).