Technical Data
Ki-67 (Antigen Identified by Monoclonal Antibody Ki-67, KIA, MKI67, Proliferation Related Ki-67 Antigen)
Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki67 index and histologically low-grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

Suitable for use in Immunofluorescence/Immunocytochemistry, Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Immunofluorescence (IC): 1:10-1:500
Western Blot: 1:100-1:2000
Immunohistochemistry (Paraffin): 1:100-1:500
Immunohistochemistry (Frozen): 1:100-1:500
Flow Cytometry: 1:100
Optimal dilution determined by the researcher.

Positive Controls:
Mouse intestine, pancreas, spleen

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ul-20CBlue IceMouseRabbit
Synthetic peptide corresponding to an internal portion (within residues 1600-1700) of mouse Ki-67 protein (Q61769). Cellular Localization: Nuclear
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris-citrate/phosphate, pH 7.5, 0.09% sodium azide.
Recognizes mouse Ki-67. Species Crossreactivity: human and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Roper, J. et al., (2011) PLoS ONE 6: e25132 (IHC).