Technical Data
K1759-24C
KIF5B (Kinesin Family Member 5B, Kinesin 5B, Kinesin 1, Kinesin Heavy Chain, KINH, KNS1, Ubiquitous Kinesin Heavy Chain, UKHC)
Description:
Kinesin belongs to the group of motor proteins known to convert chemical energy released from ATP into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120-130kD) and two light chains (60-70kD), resulting in a molecular mass about 400kD. Each heavy chain contains an N-terminal globular motordomain with both a microtubule binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.

Applications:
Suitable for use in Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgM8L527Purified
SizeStorageShippingSourceHost
100ug-20CBlue IcePorcineMouse
Concentration:
~1mg/ml
Immunogen:
Enriched fraction of porcine brain kinesin. Cellular Localization: Microtubules.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity:
Recognizes the heavy chain of conventional human kinesin associated with vesicles and with lower affinity with denaturated molecule. Recognizes kinesin bound to taxol-stabilized microtubules. Species Crossreactivity: Mouse, rat, porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Macurek, L., et al., Hybrid Hybridomics 21: 457-462 (2002).