Technical Data
K1759-24G
KIF5B, CT (Kinesin-1 Heavy Chain, Conventional Kinesin Heavy Chain, Ubiquitous Kinesin Heavy Chain, UKHC, KNS, KNS1)
Description:
Kinesin belongs to the group of motor proteins known to convert chemical energy released from ATP into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120-130kD) and two light chains (60-70kD), resulting in a molecular mass about 400kD. Each heavy chain contains an N-terminal globular motordomain with both a microtubule binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000
Western Blot: 0.2-1ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml
Immunohistochemistry: 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-QPVAVRGGGGKQV from the C-terminal of human KIF5B.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human KIF5B. Species sequence homology: canine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.