Technical Data
K1759-24G
KIF5B, CT (Kinesin-1 Heavy Chain, Conventional Kinesin Heavy Chain, Ubiquitous Kinesin Heavy Chain, UKHC, KNS, KNS1)
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Technical Data |
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K1759-24G |
KIF5B, CT (Kinesin-1 Heavy Chain, Conventional Kinesin Heavy Chain, Ubiquitous Kinesin Heavy Chain, UKHC, KNS, KNS1) |
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Description: Kinesin belongs to the group of motor proteins known to convert chemical energy released from ATP into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120-130kD) and two light chains (60-70kD), resulting in a molecular mass about 400kD. Each heavy chain contains an N-terminal globular motordomain with both a microtubule binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 0.2-1ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml Immunohistochemistry: 1-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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