Technical Data
K1759-24H
KIF5B (Kinesin-1 Heavy Chain, Conventional Kinesin Heavy Chain, Ubiquitous Kinesin Heavy Chain, UKHC, KNS, KNS1)
Description:
It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments. The antibody KN-03 well recognizes kinesin bound to Taxol-stabilized microtubules.

Applications:
Suitable for use in Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgMKN-03Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IcePorcineMouse
Concentration:
~1mg/ml
Immunogen:
Enriched fraction of porcine brain kinesin.
Purity:
Purified by Thiophilic adsorption chromatography.
Form
Supplied as a liquid in PBS, 15mM sodium azide, pH 7.4.
Specificity:
Recognizes porcine heavy chain of conventional kinesin associated with vesicles and with lower affinity with denatured molecule. Species sequence homology: human, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.