		Technical Data
K1759-24H	KIF5B (Kinesin-1 Heavy Chain, Conventional Kinesin Heavy Chain, Ubiquitous Kinesin Heavy Chain, UKHC, KNS, KNS1)

Description:
It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments. The antibody KN-03 well recognizes kinesin bound to Taxol-stabilized microtubules.

Applications:
Suitable for use in Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgM	KN-03	Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Porcine	Mouse

Concentration:

~1mg/ml

Immunogen:

Enriched fraction of porcine brain kinesin.

Purity:

Purified by Thiophilic adsorption chromatography.

Form

Supplied as a liquid in PBS, 15mM sodium azide, pH 7.4.

Specificity:

Recognizes porcine heavy chain of conventional kinesin associated with vesicles and with lower affinity with denatured molecule. Species sequence homology: human, mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.