Technical Data
K1759-24J
KIF5B (Kinesin-1 Heavy Chain, Conventional Kinesin Heavy Chain, Ubiquitous Kinesin Heavy Chain, UKHC, KNS, KNS1)
Description:
KIF5B is a kinesin. These are microtubule based motor proteins involved in the transport of organelles in eukaryotic cells. They typically consist of 2 identical, approximately 110 to 120kD heavy chains and 2 identical, approximately 60 to 70kD light chains. The heavy chain contains 3 domains: a globular N terminal motor domain, which converts the chemical energy of ATP into a motile force along microtubules in 1 fixed direction; a central alpha helical rod domain, which enables the 2 heavy chains to dimerize; and a globular C terminal domain, which interacts with light chains and possibly an organelle receptor.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000
Western Blot: 0.5-1.5ug/ml, observed in lysates of the cell lines Jurkat and HeLa on ~110kD bands
Immunohistochemistry (Paraffin): 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-QPVAVRGGGGKQV, from KIF5B, at C-terminal (NP_004512.1).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes human KIF5B. Species Crossreactivity: Canine. Species sequence homology: Bovine, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Astanina K, Jacob R. KIF5C, a kinesin motor involved in apical trafficking of MDCK cells. Cell Mol Life Sci. 2010 Apr;67(8):1331-42.