Technical Data
KIF14 (Kinesin Family Member 14, KIAA0042, Kinesin-like Protein KIF14, MGC142302)
The kinesin superfamily proteins (KIFs) are microtubule-dependent molecular motors that transport membranous organelles and protein complexes in a microtubule- and ATP-dependent manner. Cells use KIFs to tightly control the direction, destination and speed of the transportation of a variety of important functional molecules, including mRNA. KIFs are involved in neuronal function and development. Kinesin family member 14 (KIF14) is an overexpressed potential oncogene in the 1q region of genomic gain in breast cancer cell lines associated with poor prognosis breast cancer. The gain of chromosome 1q likely reflects oncogene amplification. As such KIF14 may be a potential therapeutic target and indicator of oncogenesis.

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Immunoprecipitation: 2-5ug/mg lysate
Western Blot: 1:2000-1:10,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabAffinity Purified
100ul-20CBlue IceHumanRabbit
The epitope recognized maps to a region between residue 1600 and the C-terminus (residue 1648) of human Kinesin Family Member 14 using the numbering given in entry NP_055690.1 (GeneID 9928).
Purified by immunoaffinity chromatography.
Supplied as a liquid in TBS, pH 7.2, 0.1% BSA, 0.09% sodium azide.
This antibody is specifc to the human KIF14 protein. Species Crossreactivity: Human. Other species have not been tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.