Technical Data
KIF21A (Kinesin Family Member 21A)
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml detects KIF21A in a rat brain microsomal preparation. Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with K1760-01 (2ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
200ug4C (-20C Glycerol)Blue IceMouseRabbit
Synthetic peptide corresponding to amino acids 948-963 (CKRREKLSKRREKIVKE) of mouse KIF21A with an N-terminal cysteine added for conjugation purposes (KLH coupled).
Purified by Protein A affinity chromatography.
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
Recognizes mouse KIF21A, Mr ~180kD. An unknown protein detected at Mr ~70kD was also competed by the immunizing peptide. Species Crossreactivity: rat and human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Nakamura N., et al., J. Biochem. (Tokyo) 132: 483-491, 2002 2. Marszalek J.R., et al., J. Cell Biol. 145: 469-479, 1999.