Technical Data
KIF22, phosphorylated (Ser427) (Kinesin Family Member 22, KIF-22, Kinesin-like DNA-binding Protein, Kinesin-like Protein 4, KNSL4, KID, Kinesin-like Protein KIF22, OriP Binding Protein, OBP1, OBP2, OBP, Origin of Plasmid DNA Replication Binding Protein)
KID (KNSL4; kinesin-like DNA-binding protein) is a member of the kinesin family that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis (1). KID colocalizes with mitotic chromosomes and is enriched in the kinetochore at anaphase. The N-terminal region of KID shares greater than 35% sequence homology with the motor domains of other kinesins. The C-terminal domain has limited but significant homology to NOD, a Drosophila protein and member of the kinesin family. KID also contains a putative nuclear localization signal (2). It was demonstrated that SIAH, a human homologue of the Drosophila seven in absentia (Sina), is involved in the degradation of KID via the ubiquitin-proteasome pathway (3). It was also reported that KID function is regulated by phosphorylation. Ser427 and Thr463 were identified as M phase-specific phosphorylation sites (4).

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul-20°CBlue IceHumanRabbit
Not determined
A phospho-specific peptide corresponding toresidues surrounding Serine 427 of human KIF22 .
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% Glycerol, 0.01% sodium azide, 0.05% BSA.
Recognizes human KIF22 phosphorylated on Serine 427.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Song J, et al. Genomics 52(3):374-7, 1998 2. Tokai N, et al. EMBO J. 15(3):457-67. 1996 3. Germani A, et al. Oncogene 19(52):5997-6006, 2000 4. Tokai-Nishizumi N, et al. The EMBO Journal 22: 2091-2103, 2003