Technical Data
KIF22 (Kinesin Family Member 22, KIF-22, Kinesin-like DNA-binding Protein, Kinesin-like Protein 4, KNSL4, KID, Kinesin-like Protein KIF22, OriP Binding Protein, OBP1, OBP2, OBP, Origin of Plasmid DNA Replication Binding Protein)
KID (KNSL4; kinesin-like DNA-binding protein)is a member of the kinesin family that is involved in spindleformation and the movements of chromosomes duringmitosis and meiosis (1). KID colocalizes with mitoticchromosomes and is enriched in the kinetochore atanaphase. It might play a role in regulatingthe chromosomalmovement along microtubules during mitosis. The Nterminalregion of KID shares greater than 35% sequencehomology with the motor domains of other kinesins. The Cterminaldomain has limited but significant homology to NOD,a Drosophila protein and member of the kinesin family. KID also contains a putative nuclear localization signal (2). It was demonstrated that SIAH, a human homologue of theDrosophila seven in absentia (Sina), is involved in thedegradation of KID via the ubiquitin-proteasome pathway (3).

Suitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytomtery: 1:30
Western Blot: 1:1000-1:5000
Immunoprecipitation: 1:10
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul-20°CBlue IceHumanRabbit
Not determined
A synthetic peptide corresponding to residues on the N-terminus of KIF22.
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% Glycerol, 0.01% sodium azide, 0.05% BSA.
Recognizes human KIF22.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Song J, et al. Genomics 52(3):374-7, 1998 2. Tokai N, et al. EMBO J. 15(3):457-67. 1996 3. Germani A, et al. Oncogene 19(52):5997-6006, 2000