		Technical Data
K1775	Kinase Suppressor of Ras (KSR, KSR1, BKSR1, dKsr, EK31, hb, Positive Ras Signaling Mediator Family Member, RSU2, Suppressor of Activated let 60 Ras SUR3, Suppressor of Ras1 31, SR31)

Description:
KSR was initially considered to be a protein kinase acting in the Ras pathway, but has more recently been demonstrated to act as a scaffold protein, facilitating complex formation between RAF and MEK and ERK.

Applications:
Suitable for use in Western Blot and Immunohistochemistry
Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Frozen and paraffin): Methacarn fixation recommended for paraffin embedded tissues.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Reagents:
I1904-49U IgG, F(ab’)2, H&L X-Adsorbed (FITC) RbxRt
I1904-49H IgG, F(ab’)2, H&L X-Adsorbed (HRP) RbxRt
I1904-47J IgG, H&L (AP) Pab GtxRt

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol 40-50%, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2a	3H2582	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		4°C (-20°C Glycerol)	Blue Ice	Human	Rat

Concentration:

~1mg/ml

Immunogen:

Recombinant human KSR protein.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

Specificity:

Recognizes human kinase suppressor of Ras (KSR). Species Crossreactivity: mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Morrison, D. K. et al. (2001). KSR: a MAPK scaffold of the Ras pathway? J. Cell. Sci. 114: 1609 - 1612. 2. Giblett, S. M. et al. (2002). Expression of kinase suppressor of Ras in the normal adult and embryonic mouse. Cell Growth Differentation 13: 307-313.