Technical Data
K1775-75C
Kinase-interacting Protein 2, CT (KIP 2, KIP2, Calcium and Integrin Binding Family Member 2, CIB2)
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Technical Data |
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K1775-75C |
Kinase-interacting Protein 2, CT (KIP 2, KIP2, Calcium and Integrin Binding Family Member 2, CIB2) |
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Description: DNA-dependent protein kinases (DNA-PK) play a role in the repair of double-strand DNA breaks and in the process of V(D)J recombination during lymphoid development. By EST database searching for sequences homologous to the DNA-PK gene KIP/CIB and 5-prime RACE, Seki, N et al. isolated a full-length cDNA, which they designated as KIP2, from a human fetal brain cDNA library. KIP2 encodes a deduced 187aa protein with a predicted molecular mass of 22kD. The KIP2 protein shares 46%, 39%, and 30% sequence identity with the calcium-binding proteins KIP/CIB, calcineurin B and calmodulin respectively. KIP2 contains two EF-hand motifs and a helix-loop-helix motif involved in coordinating the calcium ion, indicating that KIP2 may also bind calcium. KIP2 is ubiquitously expressed in various human tissues. Applications: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:1,000 Western Blot: 1:100-1:500 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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