Technical Data
Kinase-interacting Protein 2, NT (KIP 2, KIP2, Calcium and Integrin Binding Family Member 2, CIB2)
DNA-dependent protein kinases (DNA-PK) play a role in the repair of double-strand DNA breaks and in the process of V(D)J recombination during lymphoid development. By EST database searching for sequences homologous to the DNA-PK gene KIP/CIB and 5-prime RACE, Seki, N et al. isolated a full-length cDNA, which they designated as KIP2, from a human fetal brain cDNA library. KIP2 encodes a deduced 187aa protein with a predicted molecular mass of 22kD. The KIP2 protein shares 46%, 39%, and 30% sequence identity with the calcium-binding proteins KIP/CIB, calcineurin B and calmodulin respectively. KIP2 contains two EF-hand motifs and a helix-loop-helix motif involved in coordinating the calcium ion, indicating that KIP2 may also bind calcium. KIP2 is ubiquitously expressed in various human tissues.

Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
200ul-20CBlue IceHumanRabbit
As reported
Synthetic peptide selected from the N-terminal region of human KIP2 (KLH).
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, 0.09% sodium azide.
Recognizes human KIP2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Seki, N., et al., Biochim. Biophys. Acta 1444(1):143-147 (1999).