		Technical Data
K1879-22	Kit-a, ID (Mast/Stem Cell Growth Factor Receptor Kita, SCFR, Tyrosine-protein Kinase Kit, Kita, Kit, Sparse)

Description:
Kit receptor a (kit-a) is a receptor tyrosine kinase expressed by embryonic neural crest-derived melanocytes and their precursors who are dependent on kit's function of migration, survival, along with some melanocyte differentiation. The gene is expressed in zebrafish similarly to its mouse ortholog. However, contrary to mouse, Zf kit-a exhibits expression in primordial germ cells. Furthermore, while Zf kit-a is not required for blood development, it is expressed in a primitive hematopoiesis location.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:5000-1:20,000
Optimal dilutions to be determined by the researcher.

Control Peptide: K1879-22P

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
250ul		-20°C	Blue Ice	Zebrafish	Rabbit

Concentration:

As reported

Immunogen:

Synthetic peptide corresponding to the intermediate region of zebrafish Kit-a.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 50% glycerol.

Specificity:

Recognizes zebrafish Kit-a.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Parichy, DM, et al (1999) Development 126(15):3425-3436. 2. Rawls, JF and Johnson, SL (2001) Development 128(11):1943-1949. 3. Mellgren, EM and Johnson, SL (2005) Dev. Genes Evol. 215(9):1-8.