Technical Data
Media
Kligler Iron Agar (Powder)
K1892
Microbiological Grade
Storage RT    Shipping RT
Formula grams/Liter
Enzymatic Digest of Casein10
Enzymatic Digest of Animal Tissue10
Lactose10
Dextrose1
Ferric Ammonium Citrate0.5
Sodium Chloride5
Sodium Thiosulfate0.5
Phenol Red0.025
Agar15
Total52g/liter
Differential medium for identification of enteric bacteria based on fermentation of dextrose, lactose and H2S production. This medium permits differentiation of Gram-negative bacilli by their ability to ferment Dextrose or Lactose, and produce hydrogen sulfide. Enzymatic Digest of Casein and Enzymatic Digest of Animal Tissue provide nitrogen, carbon, and vitamins required for organism growth. Ferric Ammonium Citrate and Sodium Thiosulfate are indicators of hydrogen sulfide production. Phenol Red is the pH indicator. Sodium Chloride maintains the osmotic balance of the medium. Agar is the solidifying agent.


Directions for preparation: 1. Suspend 52 g of the medium in one liter of purified water.2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.

3. Distribute into test tubes and autoclave for 15 minutes at 121°C. Storage and Stability:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Form: Supplied as a light beige, free-flowing homogeneous powder.

Solution: Prepared medium is reddish-orange to red, trace to slightly hazy.

pH: 7.4


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.