Technical Data
Kligler Iron Agar (Powder)
Microbiological Grade
Storage RT    Shipping RT
Formula grams/Liter
Enzymatic Digest of Casein10
Enzymatic Digest of Animal Tissue10
Ferric Ammonium Citrate0.5
Sodium Chloride5
Sodium Thiosulfate0.5
Phenol Red0.025
Differential medium for identification of enteric bacteria based on fermentation of dextrose, lactose and H2S production. This medium permits differentiation of Gram-negative bacilli by their ability to ferment Dextrose or Lactose, and produce hydrogen sulfide. Enzymatic Digest of Casein and Enzymatic Digest of Animal Tissue provide nitrogen, carbon, and vitamins required for organism growth. Ferric Ammonium Citrate and Sodium Thiosulfate are indicators of hydrogen sulfide production. Phenol Red is the pH indicator. Sodium Chloride maintains the osmotic balance of the medium. Agar is the solidifying agent.

Formulation (g/L):
Enzymatic Digest of Casein 10
Enzymatic Digest of Animal Tissue 10
Lactose 10
Dextrose 1
Ferric Ammonium Citrate 0.5
Sodium Chloride 5
Sodium Thiosulfate 0.5
Phenol Red 0.025
Agar 15
Total: 52g/L

Directions per Liter:
1. Suspend 52g of the medium in one liter of ddH2O.
2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
3. Distribute into test tubes and autoclave for 15 minutes at 121C.
4. After autoclaving, allow medium to solidify in a slanted position.

Storage and Stability:
Store sealed bottle containing the dehydrated medium at RT. Once opened and recapped, place container in a low humidity environment at the same storage temperature. Protect from moisture and light.

Directions for preparation: 1. Suspend 52 g of the medium in one liter of purified water.2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.

3. Distribute into test tubes and autoclave for 15 minutes at 121C. Storage and Stability:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Form: Light beige, free-flowing homogeneous powder

Solubility: Reddish-orange to red, trace to slightly hazy

pH (5.2%): 7.4

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Kligler, I. J. 1917. A simple medium for the differentiation of members of the typhoid-paratyphoid group. Am. J. Public Health 7:1042-1044. 2. Russell, F. F. 1911. The isolation of typhoid bacilli from urine and feces with the description of a new double sugar tube medium. J. Med. Res. 25:217. 3. Kligler, I. J. 1918. Modifications of culture media used in the isolation and differentiation of typhoid, dysentery, and allied bacilli. J. Exp. Med. 28:319-322. 4. Bailey, S. F., and L. R. Lacy. 1927. A modification of the Kligler lead acetate medium. J. Bacteriol. 13:183. 5. Sulkin, S. E., and J. C. Willett. 1940. A triple sugar-ferrous sulfate medium for use in identification of enteric organisms. J. Lab. Clin. Med. 25:649-653. 6. Isenberg, H. D. (ed.). 1992. Clinical microbiology procedures handbook, vol.1. American Society for Microbiology, Washington, D.C. 7. Murray, P. R., E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (eds.). 1995.Manual of clinical microbiology, 6th ed. American Society for Microbiology, Washington, D. C. 8. Bacteriological Analytical Manual. 1995. 8th ed. AOAC International, Gaithersburg, M.D. 9. MacFaddin, J. F. 1985. Media for isolation-cultivation-identification-maintenance of medial bacteria, Williams & Wilkins, Baltimore, MD.