Technical Data
K1894
KpnI
4000u
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Technical Data |
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K1894 |
KpnI |
4000u |
| Molecular Biology | Storage: -20°CShipping: Blue Ice |
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5'-G G T A C^C-3' 3'-C^C A T G G-5' Concentration: 10u/ul Source: HC Klebsiella pneumoniae OK8 Supplied with: R1625-65: Restriction Enzyme Buffer for KpnI, 10X 10mM Tris-HCl (pH 7.5), 10mM MgCl2, 0.02% Triton X-100 and 0.1mg/ml BSA. Incubate at 37°C Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol, or Storage Buffer. Storage Buffer: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol. |
Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion (5u/ug lambda DNA x 16 hours) with KpnI (see Star Activity). Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with KpnI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.02uM. More than 95% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours. Blue/White Cloning Assay: pUC57 was digested at a unique site with 10 units of enzyme for 5 hours. After religation and transformation 0.1% of white colonies were detected. Star Activity: A large excess of enzyme (10u/ug DNA x 16 hours) may result in star activity. Stability during Prolonged Incubation: A minimum of 0.2 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C. Thermal Inactivation: Enzyme is inactivated by incubation at 80°C for 20min. Digestion of Agarose-embedded DNA: A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Number of Recognition Sites in DNA: |
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