Technical Data
K6850
KSR (Kinase Suppressor of Ras, KSR1, BKSR1, dKsr, EK31, hb, Positive Ras Signaling Mediator Family Member, RSU2, Suppressor of Activated let 60 Ras SUR3, Suppressor of Ras1 31, SR31)
Description:
KSR was initially considered to be a protein kinase acting in the Ras pathway, but has more recently been demonstrated to act as a scaffold protein, facilitating complex formation between RAF and MEK and ERK.

Applications:
Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: Endogenous levels of KSR may be too low to detect by Western Blot. Immunoprecipitation prior to Western Blot is recommended for detection of endogenous KSR. Note levels of recombinant KSR in transfected cells may be high enough to be detected by Western Blot.
Immunoprecipitation/Western Blot: 4ug/ml detects KSR by Western Blot after using 10ug of this lot to immunoprecipitate KSR from 1mg of 3T3/A31 RIPA lysate. 3T3/A31 cell lysate was immunoprecipitated with 10ug of K6850 and immune complexes were resolved by electrophoresis, transferred to nitrocellulose and probed with K6850 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Other bands correspond to light chain, heavy chain, and heavy chain dimer. The band above KSR may be incompletely reduced antibody, as it was present in a nonspeci c antibody control.
Optimal dilutions to be determined by the researcher.

Recommended secondary antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG4i45Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
GST fusion protein corresponding to residues 118-248 of rat KSR.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
Specificity:
Recognizes rat KSR, Mr 120kD. Species Crossreactivity: human and mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Cacace, A.M., et al., Mol. Cell Biol. 19: 229240, 1999.