Technical Data
K6850-01A
KSR (Kinase Suppressor of Ras, KSR1, BKSR1, dKsr, EK31, hb, Positive Ras Signaling Mediator Family Member, RSU2, Suppressor of Activated let 60 Ras SUR3, Suppressor of Ras1 31, SR31)
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Technical Data |
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K6850-01A |
KSR (Kinase Suppressor of Ras, KSR1, BKSR1, dKsr, EK31, hb, Positive Ras Signaling Mediator Family Member, RSU2, Suppressor of Activated let 60 Ras SUR3, Suppressor of Ras1 31, SR31) |
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Description: The Kinase Suppressor of Ras (KSR1) is a recently identified participant in the Ras signaling pathway. KSR is capable of uncoupling the MAP kinase activation from its target phosphorylation, and thus provide a novel mechanism for modulating the Ras-MAP kinase signaling pathway. The recently identified, B-KSR, is a related isoform. KSR is thought to phosphorylate and activate Raf-1, protecting against cytokine-mediated apoptosis. Recent studies suggest that KSR interacts with several components of the MAP kinase pathway, including Raf-1, MEK1/2 and ERK1/2. KSR seems to act as a scaffolding protein that coordinates the assembly of a membrane-localized, multiprotein MAP kinase complex, a vital step in Ras-mediated signal transduction. Applications: Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry (Paraffin). Other applications not tested. Recommended Dilution: Western Blot Analysis: 1:500-1:2000 Immunocytochemistry: 1:50 Immunohistochemistry (Paraffin): 1:100-250 Optimal dilutions to be determined by the researcher. Positive Control: 293 cell lysate. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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