Description:
The Ku autoantigen is a heterodimer of 70kD (p70) and ~80kD (p80) proteins. The p70/p80 dimer is important for function of a 460kD DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
Applications:
Suitable for use in Immunoprecipitation, Flow Cytometry, Immunofluorescence and Immunohistochemistry. Not suitable for Western Blot. Optimal dilutions to be determined by the researcher.
Recommended Dilution:
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.
Immunoprecipitation: Tested only on mouse and rat
Optimal dilutions to be determined by the researcher.
Recommended Positive Control:
Tonsil
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG2a | 3F247 | Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 500ul | | -20°C | Blue Ice | Human | Mouse |
|
| Concentration: |
| ~0.2mg/ml |
| Immunogen: |
| Human B cell nuclei from plasmacytoid 2p68 cells. Cellular Localization: Nuclear. Epitope: A conformational epitope of p70/p80 dimer, which is destroyed during Western Blot. |
| Purity: |
| Purified by ammonium sulfate precipitation. |
| Form |
| Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See K7965-05X |
| Specificity: |
| Recognizes human Ku, p70, p80. Species Crossreactivity: monkey, and xenopus. Mouse and rat (Immunoprecipitation only). Does not react with bovine and rabbit. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Wang J, et. al. Molecular Biology Reports, 1993, 18(1):15–28. 2. Reeves WH, et. al. Journal of Experimental Medicine, 1985, 161(1):18–39.
1. Griffin W,et al.. Sequence-specific DNA binding by autoantigen and its effects on transcription. Nature, 1996, 380:265–8. 2. Ajmani AK; et al Journal of Experimental Medicine, 1995 Jun 1, 181(6):2049–58. 3. Boubnov NV; et al. Proceedings of the National Academy of Sciences of the United States of America, 1995 Jan 31, 92(3):890–4. 4. DiCroce PA; et al Proceedings of the National Academy of Sciences of the United States of America, 1995 Oct 24, 92(22):10137–41. 5. Wang J et al. Molecular Biology Reports, 1993 Jun, 18(1):15–28. 6. Reeves WH. Journal of Experimental Medicine, 1985 Jan 1, 161(1):18–39.
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