The Ku autoantigen is a heterodimer of 70kD (p70) and ~80kD (p80) proteins. The p70/p80 dimer is important for function of a 460kD DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
Suitable for use in Immunoprecipitation, Flow Cytometry, Immunofluorescence and Immunohistochemistry. Not suitable for Western Blot. Optimal dilutions to be determined by the researcher.
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.
Immunoprecipitation: Tested only on mouse and rat
Optimal dilutions to be determined by the researcher.
Recommended Positive Control:
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Human B cell nuclei from plasmacytoid 2p68 cells. Cellular Localization: Nuclear. Epitope: A conformational epitope of p70/p80 dimer, which is destroyed during Western Blot.|
|Purified by ammonium sulfate precipitation.|
|Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See K7965-05X|
|Recognizes human Ku, p70, p80. Species Crossreactivity: monkey, and xenopus. Mouse and rat (Immunoprecipitation only). Does not react with bovine and rabbit.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Wang J, et. al. Molecular Biology Reports, 1993, 18(1):15–28. 2. Reeves WH, et. al. Journal of Experimental Medicine, 1985, 161(1):18–39. |
1. Griffin W,et al.. Sequence-specific DNA binding by autoantigen and its effects on transcription. Nature, 1996, 380:265–8. 2. Ajmani AK; et al Journal of Experimental Medicine, 1995 Jun 1, 181(6):2049–58. 3. Boubnov NV; et al. Proceedings of the National Academy of Sciences of the United States of America, 1995 Jan 31, 92(3):890–4. 4. DiCroce PA; et al Proceedings of the National Academy of Sciences of the United States of America, 1995 Oct 24, 92(22):10137–41. 5. Wang J et al. Molecular Biology Reports, 1993 Jun, 18(1):15–28. 6. Reeves WH. Journal of Experimental Medicine, 1985 Jan 1, 161(1):18–39.