Technical Data
K9003-10
Kv1.5 (Kcna5, Voltage-gated, Delayed-rectifier Potassium Channel)
Description:
Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potasssium ions may pass in accordance with their electrochemical gradient. May play a role in regulating the secretion of insulin in normal pancreatic islets.

Potassium channels represent the most complex class of voltage-gated ino channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes-shaker, shaw, shab, and shal-have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class, the function of which could restore the resting membrane potential of beta cells after depolarization and thereby contribute to the regulation of insulin secretion. This gene is intronless, and the gene is clustered with genes KCNA1 and KCNA6 on chromosome.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:200-1:400 (Rat brain membranes).
Immunohistochemistry: Rat brain sections
Note: Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal dilution determined by the researcher.

Epitope:
HRETD HEEQA ALKEE QGIQR RESGL DTGGQ RKVSC SKASF HKTGG PLEST DSIRR GSCPL EKCHL KAKSN VDLRR SLYAL CLDTS RETDL

Control Antigen Included:
K9003-10A1

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ul-20CBlue IceMouseRabbit
Concentration:
~0.5mg/ml
Immunogen:
GST Fusion protein and a C-terminal portion (aa513-602) of mouse Kv1.5 (Accession # Q61762). Epitope: HRETD HEEQA ALKEE QGIQR RESGL DTGGQ RKVSC SKASF HKTGG PLEST DSIRR GSCPL EKCHL KAKSN VDLRR SLYAL CLDTS RETDL
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 5% sucrose, 0.05% sodium azide.
Specificity:
Recognizes mouse Kv1.5. Species Crossreactivity: Rat, equine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hu, X.Q., et al., J. Biol. Chem. 273: 5337 (1998). 2. Guo, W., et al., Am. J. Physiol. 272: H2599 (1997). 3. Guo, W., et al., Eur. J. Physiol. 434: 206 (1997). 4. Clement-Chomienne, O., et al., J. Physiol. 515.3: 653 (1999). 5. Sobko, A., et al., J. Neuroscience 18: 10,398 (1998). 6. Sobko, A., et al., EMBO J. 17: 4723 (1998). 7. Maruoka, N.D., et al., FEBS Letters 473: 188 (2000). 8. Yamashita, T., et al., Circulation 101: 2007 (2000). 9. Chung, Y.H., et al., Brain Res. 875: 164 (2000). 10. Peretz, A., et al., EMBO J. 19: 4036 (2000).