Technical Data
L0899
LacI
Description:
The lac repressor protein (Lac I) is an important genetic control protein. It controls the expression of the lactose metabolizing enzymes in Escherichia coli. This regulation involves specific recognition of the operator DNA sequence (Lac O) and consequent inhibition of transcription initiation by RNA polymerase; modulation of binding at this target site by small sugar molecules, inducers, provides the basis for regulation. Different ligand binding activities are associated with the isolated domains of Lac I: specific and nonspecific DNA binding with the NH2 termini, and inducer and specific DNA binding with the core protein.

Applications:
Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 0.5-2ug/ml. Detects LacI from LacI transfected Cos-1 whole cell lysate.
Immunocytochemistry: Reported to show nuclear immunostaining in LacI transfected cells fixed with methanol.
Immunohistochemistry: Reported to show nuclear immunostaining in LacI transgenic tissue fixed with 4% paraformaldehyde and permeabilized with Triton X-100.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG10.T.81Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant TrpE-fusion protein containing full length E. coli LacI.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.0, 0.01M EDTA.
Specificity:
Recognizes E. coli LacI at 38kD and recombinant proteins containing a LacI tag.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Scrable, H. and Stambrook, P.J., Genetics 147: 297-304 (1997).