Technical Data
Media
Lactobacillus Selective Agar Base (Powder)
L1021-50
Microbiological Grade
Storage RT/4C    Shipping RT
Components g/liter
Enzymatic Digest of Casein 10
Yeast Extract 5
Monopotassium Phosphate6
Ammonium Citrate 2
Dextrose 20
Sodium Acetate Hydrate 25
Magnesium Sulfate 0.575
Manganese Sulfate 0.12
Ferrous Sulfate 0.034
Polysorbate 80 1
Agar 15
Total84.7
Selective medium for isolation of Lactobacilli from meats, other foods and dairy products.

Selective medium for isolation of Lactobacilli from meats, other foods and dairy products. Enzymatic digest of casein provides carbon, nitrogen, and amino acids used to support general growth requirements in Lactobacillus Selective Agar Base. Yeast Extract is a major source of vitamins. Dextrose is a carbohydrate. Sodium acetate hydrate and ammonium citrate inhibit streptococci, molds, and other oral microbial flora and restrict swarming. Monopotassium phosphate is the buffering agent. Magnesium sulfate, manganese sulfate and ferrous sulfate are sources of inorganic ions. Polysorbate 80 acts as a surfactant. Agar is the solidifying agent.

Microorganism Growths:
Escherichia coli - Inhibited
Lactobacillus casei - Growth
Lactobacillus fermentum - Growth
Lactobacillus plantarum - Growth
Staphylococcus aureus - Inhibited

Storage:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.


Directions 1. Suspend 84.7g of the medium in one liter of purified water. Mix thoroughly. 2. Add 1.32 mL of glacial acetic acid. 3. Heat with frequent agitation and boil for one minute to completely dissolve the medium.

4. Avoid overheating. Do not autoclave.. Storage and Stability:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: Supplied as a homogeneous, free flowing beige powder.

Solubility: Slightly hazy, light beige to amber solution.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Rogosa, M., J. A. Mitchell, and R. F. Wiseman. 1951. A selective medium for the isolation and enumeration of oral and fecal lactobacilli. J. Bacteriol. 62:132. 2. Rogosa, M., J. A. Mitchell, and R. F. Wiseman. 1951. A selective medium for the isolation and enumeration of oral and fecal lactobacilli. J. Dental Res. 30:682. 3. Vedamuthu, E. R., M. Raccach, B. A. Glatz, E. W. Seitz, and M. S. Reddy. 1992. Acid-producing microorganisms. In C. Vanderzant, and D. F. Splittstoesser (eds.). Compendium of methods for the microbiological examination of foods, 3rd ed. American Public Health Association, Washington, D.C.