Technical Data
L1021-75D
Lactoferrin (LTF, LF, GIG12, HLF2, Homo sapiens Chemokine Receptor X, CKRX, Kaliocin 1, Lactoferroxin A, Lactoferroxin B, Lactoferroxin C, Lactotransferrin, Talalactoferrin alfa)
Description:
Lactoferrin is an iron binding glycoprotein with an approximate molecular weight of 80kD. The protein has two iron binding domains each housing one Fe 3+ and the synergistic CO 3 2- ion. The crystal structure form of human lactoferrin at 2.2A resolution exhibits 5330 protein atoms, 2Fe2+, 2CO 32- and 98 carbohydrate atoms. Lactoferrin is absorbed from intestine by apical side of the membrane and localized to the nuclei. Intravenous infusion of lactoferrin is protective against lethal doses of E. coli and induce bacterimia by a mechanism that downregulates neutrophil TNF alfa secretion. Recombinant human lactoferrin (rhLF), expressed and extracted from rice seed, is being evaluated for use as a dietary supplement to treat iron deficiency and/or iron deficiency induced anemia. Lactoferrin has been shown to have a role in the immune system and in early development of the embryo. A specific receptor for lactoferrin binding has been implicated in the human fetal intestine. Early embryonic localization of lactoferrin by IHC has suggested its presence in various tissues including intestinal epitheliuem, kiney, and various regions of the brain.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen and paraffin): 1:200 Paraffin-embedded sections require some antigen retrieval.
Immunoprecipitation: >1:200
Western Blot: >1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Full length purified, native human Lactoferrin. Cellular Localization: Secreted
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid. No preservatives added.
Specificity:
Recognizes human lactoferrin at ~79kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ashida K et al. Cellular internalization of lactoferrin in intestinal epithelial cells. Biometals 17:311-5 (2004). 2. Zimecki M et al. Protective effects of lactoferrin in Escherichia coli-induced bacteremia in mice: relationship to reduced serum TNF alpha level and increased turnover of neutrophils. Inflamm Res 53:292-6 (2004). 3. Sharma AK Purification, crystallization and preliminary crystallographic analysis of mare lactoferrin. Acta Crystallogr D Biol Crystallogr 52:1196-8 (1996). 4. Haridas M Structure of human diferric lactoferrin refined at 2.2 A resolution. Acta Crystallogr D Biol Crystallogr 51:629-46 (1995). .