Technical Data
L1169-05B
Lambda Chain (B cells)
Description:
Human immunoglobulins are glycoproteins composed of two disulfide-bonded heavy (H) chain subunits, each of which is linked by interchain disulfide bonds to a light (L) chain forming a tetramolecular complex. There are five classes of immunoglobulins, designated IgG, IgA, IgM, IgD and IgE, which are defined by differences in the constant region of H chains. L chains are divided into kappa or lambda classifications based on structural antigenic differences. All classes of immunoglobulins have been found on the cell surface of B lymphocytes where they function as antigen receptors to elicit antigen-dependent proliferation and secretion of antigen specific soluble circulating antibodies.

Applications:
Suitable for use in Flow Cytometry and ELISA. Other applications not tested.

Recommended Dilution
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG17H194Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human Ig
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in FBS, 50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate.
Specificity:
Recognizes cell surface lambda chain on B cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Basic and Clinical Immunology, Seventh edition (D. P. Sites & A. I. Terr, eds.) Appleton & Lange., Norwalk, CT (1991).