Technical Data
L1185-05X
Lambda Light Chain (BSA & Azide Free)
Description:
Antibody to the lambda light chain of immunoglobulin is reportedly useful in the identification of leukemias, plasmacytomas and certain non-Hodgkins lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin/Paraffin Sections): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Positive Control:
Tonsil

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a,k0.N.379Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified human IgG myeloma proteins covalently coupled to polyaminostyrene microbeads. Cellular Localization: Cytoplasmic. MW of Antigen: 25kD.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See L1185-05.
Specificity:
Recognizes the lambda light chains of human immunoglobulins. Also recognizes free and Bence-Jones lambda light chains. Does not crossreact with kappa light chains. Species Crosseactivity: Does not react with rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Reimer, C.B., et al., Hybridoma 3: 263-275 (1984). 2. Black, C.M., et al., Immunol. Lett. 37(2-3): 207-213 (1993). 3. Jefferis, R., et al., Immunol. Lett. 31(2): 143-168 (1992). 4. Phillips, D.J., et al., Immunol. Lett. 17(2): 159-168 (1988). 5. Jefferis, R., et al., Immunol. Lett. 10(3-4): 223-252 (1985).