Technical Data
Lambda Light Chain
This antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single light chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (Formalin/paraffin): 1:100-1:200 for 30 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Positive Control: Tonsil

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
500ul4C (-20C Glycerol)Blue IceHumanRabbit
Not determined.
Lambda light chains purified from human Bence Jones proteins. Cellular Localization: Cytoplasmic. MW of antigen: 25kD
Supplied as liquid in PBS, pH 7.4, 0.09% sodium azide, 0.2% BSA. Also supplied without BSA and sodium azide. See L1185-07X.
Recognizes lambda light chains of human immunoglobulins, free lambda light chains and Bence-Jones lambda light chains (25kD). Does not crossreact with kappa light chains. Species Crossreactivity: Human, baboon, mouse and rat. Does not crossreact with canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.