Technical Data
L1185-07
Lambda Light Chain
Description:
This antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single light chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Applications:
Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (Formalin/paraffin): 1:100-1:200 for 30 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Positive Control: Tonsil

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 24 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
500ul4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined.
Immunogen:
Lambda light chains purified from human Bence Jones proteins. Cellular Localization: Cytoplasmic. MW of antigen: 25kD
Purity:
Purified
Form
Supplied as liquid in PBS, pH 7.4, 0.09% sodium azide, 0.2% BSA. Also supplied without BSA and sodium azide. See L1185-07X.
Specificity:
Recognizes lambda light chains of human immunoglobulins, free lambda light chains and Bence-Jones lambda light chains (25kD). Does not crossreact with kappa light chains. Species Crossreactivity: Human, baboon, mouse and rat. Does not crossreact with canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.