Technical Data
L1185-11A
Lambda Light Chain
Description:
Recommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG HRP, F(ab')2 rabbit anti mouse IgG: RPE conjugate

Fusion Partners: Spleen cells of immunized mice were fused with cells of the P3-X63-Ag 8.653 mouse myeloma line.

Applications:
Flow Cytometry: 1:10-1:30
Immunohistochemistry: (frozen)- Neat-1:10, (paraffin)- Neat-1:10
ELISA: 1:100
Optimal working dilutions to be determined by researcher.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Method sheets are available on request
Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin sections.
TypeIsotypeCloneGrade
MabIgG2a3H2590Supernatant
SizeStorageShippingSourceHost
2ml-20CBlue IcePorcineMouse
Concentration:
As reported
Immunogen:
Porcine IgG.
Purity:
Tissue Culture Supernatant.
Form
Supplied as a liquid in 0.2M Tris HCl pH 7.4, 5-10% fetal calf serum, 0.09% sodium azide.
Specificity:
Porcine Ig lambda light chain.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sinoka, J. et al. (2001). Characterisation of monoclonal antibodies recognising kappa and lambda chains in pigs by flow cytometry. Vet. Immunol. Immunopathol. 80: 79 - 91