Description:
Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
FITC: Protein (molar ratio): 3.5: 1
Recommended Negative Controls: Mouse IgG1 Negative Control: FITC
Applications:
Flow Cytometry: Neat
Optimal working dilutions to be determined by researcher.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells or 100ul washed whole blood.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG1 | 3H2592 | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 100ug | | -20°C | Blue Ice | Human | Mouse |
|
| Concentration: |
| 100 tests |
| Immunogen: |
| Purified human IgG. |
| Purity: |
| Purified by Protein A affinity chromatography. |
| Form |
| Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. |
| Specificity: |
| Recognizes human lambda light chains and shows no cross- reactivity to kappa light chains or to immunoglobulin heavy chains. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Reynolds, W. M. et al. (1992) A simple technique for the determination of kappa and lambda immunoglobulin light chain expression by B cells in whole blood : J. Imm. Meth. 151:123-129
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