Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
FITC: Protein (molar ratio): 3.5: 1
Recommended Negative Controls: Mouse IgG1 Negative Control: FITC
Flow Cytometry: Neat
Optimal working dilutions to be determined by researcher.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells or 100ul washed whole blood.
| 100 tests|
|Purified human IgG.|
|Purified by Protein A affinity chromatography.|
|Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.|
|Recognizes human lambda light chains and shows no cross- reactivity to kappa light chains or to immunoglobulin heavy chains. |
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Reynolds, W. M. et al. (1992) A simple technique for the determination of kappa and lambda immunoglobulin light chain expression by B cells in whole blood : J. Imm. Meth. 151:123-129|