Technical Data
L1225-35
Laminin 5 (Laminin gamma 2)
Description:
Laminins are basement membrane components thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin 5 is a complex glycoprotein composed of three subunits (alpha, beta, and gamma). Laminin 5 is thought to be involved in cell adhesion, signal transduction and differentiation of keratinocytes. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa. Alternatively spliced transcript variants encoding different isoforms have been identified.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Inhibition. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500-1:2000 detects Laminin 5 on 10ug of human recombinant Laminin.
Immunohistochemistry (Paraffin): 5-10ug/ml; Do not over fix. A light 4% or 2% PFA is best. 4mm thick paraffin sections are dewaxed, rehydrated and immersed in 0.3% hydrogen peroxide-containing methanol for inactivation of intrinsic peroxidase, followed by treatment with Protease XXIV for 15 minutes at RT. Incubate at 37C for 1 hour, followed by secondary antibody detection and visualize with DAB.
Inhibition: 40ug/ml inhibits a2b1 integrin binding to laminin 5. Optimal dilutions to be determined by the researcher.

Recommended Controls:
Human pancreatic tumor tissue, human breast carcinoma tissue, human placenta lysates, A431 whole cell lysates.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12Q586Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
GST fusion protein expressing aa382-608 of human laminin gamma 2 chain (III domain) (NP_005553).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Specificity:
Recognizes the 150kD (precursor form) and 105kD (mature form) chain proteins of human Laminin 5. No reactivity is expected with other laminin types, as the gamma 2 chain of laminin is unique to Laminin 5. Strong positive staining is seen in basement membrane of the following tissues: skin, esophagus, lung and kidney. Positive staining is also seen in stomach, small intestine, spleen, thymus, prostate and ovary. Thyroid, testis, skeletal and cardiac muscle were negative for Laminin 5 staining.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological Application Reference: 1. El-Domyati, M., et al., Egypt J. Derm. & Androl. 29: 81-90 (2009). General References: 1. Mizushima, H., et al., Hormone Research 50: 7-14 (1998). 2. Koshikawa, N., et al., Cancer Res. 59: 5596-5601 (1999). 3. Decline, F. and Rousselle, P., J. of Cell Sci. 114: 811-823 (2000). 4. Neurieiter, D., et al., Pancreatology 5: 387-398 (2005). 5. Petty, A.P., et al., Am. J. Pathol. 170: 1763-1780 (2007).