		Technical Data
L1226-10	Laminin B1, beta1

Description:
Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Epithelial sheets in vivo are separated from the mesenchymal elements of the stroma by a thin layer of a specialized type of extracellular matrix termed the basement membrane (BM). This structure consists of individual components, some of which are ubiquitous in BMs and some are not. The ubiquitous ones comprise laminin (LN), entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms.

Applications:
Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunoprecipitation (Native and denatured): 2ug antibody/mg protein lysate using Protein G.
Western Blot: 1-2ug/ml for 2 hrs at RT.
Immunohistochemistry: 2ug/ml for 30 min at RT (Acetone-fixed frozen and alcohol-fixed, paraffin-embedded).
Optimal dilutions to be determined by the researcher.

Positive Control:
LS174T cells. Normal colon or colon carcinoma.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1,k	0.N.383 (LT3)	Affinity Purified

Size		Storage	Shipping	Source	Host
500ul		-20°C	Blue Ice	Mouse	Rat

Concentration:

~0.2mg/ml

Immunogen:

Murine EHS laminin preparation. Cellular Localization: Basement membrane.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide. Also available without BSA and azide. See L1226-10X.

Specificity:

Recognizes the laminin B chain at ~210kD. Stains most human and mouse basement membranes. Does not stain glomerular and arterial membranes. Species Crossreactivity: Human, porcine, hamster and mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Ljubimov, A.V., et al., Exp. Cell Res. (1986) 165: 530-540. 2. Ljubimov, A.V., et al., Lab Investigation (1995) 72: 461-473. 3. Couchman, J.R., et al., Matrix (1989) 9: 311-321.