Technical Data
L1227-61
Laminin 5, Human (Laminin gamma 2)
10ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
Laminins are basement membrane components thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. laminin 5 is a complex glycoprotein composed of three subunits (alpha, beta, and gamma). Laminin 5 is thought to be involved in cell adhesion, signal transduction and differentiation of keratinocytes. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa. Alternatively spliced transcript variants encoding different isoforms have been identified.

Laminin 5, from human foreskin keratinocytes (conditioned culture supernatant).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: Non reducing conditions
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Human foreskin keratinocytes
Purity: ~95% (SDS-PAGE). Purified by immunoaffinity chromatography using L1225-35A.
Concentration: ~0.2mg/ml
Form: Supplied as a liquid in PBS, pH 7.4. No preservative added.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Tsunenaga, M., et al., (1998), "Laminin 5 can promote assembly of the lamina densa in the skin equivalent model", Matrix Biol., Dec; 17(8-9), 603-613.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.