Technical Data
LAT (Linker for activation of T Cells)
LAT (linker for activation of T-cells) is involved in the T-cell antigen receptor (TCR) signal transduction pathway, and may play an important role downstream in activating protein tyrosine kinases (1). LAT is phosphorylated by ZAP-70/Syk protein tyrosine kinases leading to recruitment of multiple signaling molecules (2). It has been found that Tyr191 and Tyr171 are required for T-cell activation and Tyr132 is required for the activation of PLCgamma1 and Ras signaling pathways, respectively. Tyr226 and Tyr191 are required for Vav binding, whereas Tyr171 and Tyr132 are necessary for association and activation of phosphoinositide 3-kinase activity and PLCgamma1 (3).

Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:40
Western Blot: 1:500
Immunohistochemistry: 1:100
Immunoprecipitation: 1:60

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul -20°CBlue IceHumanRabbit
A synthetic peptide corresponding to residues surrounding GRB2-binding motif 2 of human LAT
Supplied as a liquid.
Recognizes human LAT
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Weber, J.R., et al. (1998) J Exp Med. 187:1157–61. 2. Zhang, W., et al. (1998) Cell 92:83–92. 3. Paz, P.E., et al. (2001) Biochem J. 356(Pt 2):461–71.