Technical Data
L1390-01F
LAT (Linker for Activation of T Cells, Linker for Activation of T cells Family Member 1, LAT1, 36kD Phospho-tyrosine Adapter Protein, p36,38, pp36)
Description:
Linker for activation of T cells (LAT) is an integral membrane protein (36 to 38kD), which plays an important role in linking engagement of the TCR to the biochemical events of T cell activation. LAT antibody stains thymus and peripheral lymphoid tissues such as T cell areas in lymph nodes and spleen. LAT is expressed in T lymphocytes in interstitial spaces, platelets and megakaryocytes. LAT is not expressed in B cells, macrophages, plasma cells, monocytes, epithelial histiocytes and dendritic cells.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide: GAPDYENLQELN, representing the C-Terminus of the human protein (residues 251-262), according to NP_055202.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:
Species Crossreactivity: Crossreacts with Human. Expected based upon sequence homology: rat, mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Finco TS et al. LAT is required for TCR-mediated activation of PLCgamma1 and the Ras pathway. Immunity 9:617-26 (1998).