Technical Data
LAT, phosphorylated (Tyr200) (Linker for Activation of T Cells, Linker for Activation of T cells Family Member 1, LAT1, 36kD Phospho-tyrosine Adapter Protein, p36,38, pp36)
LAT (linker for activation of T-cells) is involved in the T-cell antigen receptor (TCR) signal transduction pathway, and may play an important role downstream in activating protein tyrosine kinases (1). LAT is phosphorylated by ZAP-70/Syk protein tyrosine kinases leading to recruitment of multiple signaling molecules (2). It has been found that Tyr191 and Tyr171 (on the short isoform of LAT) are required for T-cell activation and Tyr132 is required for the activation of PLCgamma1 and Ras signaling pathways, respectively. Tyr226 and Tyr191 are required for Vav binding, whereas Tyr171 and Tyr132 are necessary for association and activation of phosphoinositide 3-kinase activity and PLCgamma1. Tyr200 and Tyr220 on long isoform of LAT correspond to Tyr171 and Tyr191 on short isoform respectively (3).

Suitable for use in Immunocytochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 1:100-1:250
Western Blot: 1:5000-1:10,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul-20°CBlue IceHumanRabbit
Not determined
A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 200 of human LAT (long isoform).
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% Glycerol, 0.01% sodium azide, 0.05% BSA.
Recognizes human LAT phosphorylated on Tyrosine 200 of long isoform and Tyrosine 171 of short isoform.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Weber, J.R., et al. J Exp Med. 187: 1157 2. Zhang, W., et al. Cell 92: 83 3. Paz, P.E., et al. Biochem J. 356(Pt 2): 461-71 (2001).