Technical Data
LAT (36kD Phospho-tyrosine Adapter Protein, LAT1, Linker for Activation of T-cells Family Member 1, p36-38, pp36)
LAT (Linker for activation of T cells; also pp36) is a 36-38kD type III (no signal sequence; extracellular N-terminus) transmembrane adaptor phosphoprotein. It is expressed on mast cells, NK cells, monocytes (but not macrophages), platelets and T cells. LAT is phosphorylated upon ligation of the TcR, GPVI, and FceRI, and serves as an anchor for several SH2-domain containing signaling proteins, including PLCg1, SLP76 and Grb2. Human LAT is 262aa in length. It contains a four aa extracellular segment with a 235aa cytoplasmic domain (aa28-262). Palmitoylation occurs on Cys26 and 29. There are 13 Ser/Thr phosphorylation sites plus five Tyr sites. Multiple potential splice variants are reported. There is an alternative start site 36aa upstream of the standard site that may be accompanied by a deletion of aa114-142. There is also a deletion of aa88-97, and three substitutions: two aa for aa215-262, 38aa for aa55-262, and 50aa for aa35-262. Over aa30-113, human LAT shares 73% aa identity with mouse LAT.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanSheep
As reported
Purified, E. coli-derived recombinant human LAT (aa30-113; Accession #O43561).
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute in PBS, 0.02% sodium azide.
Recognizes human LAT.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.