		Technical Data
L1390-05E	LAT, CT (Linker for Activation of T Cells, Linker for Activation of T cells Family Member 1, LAT1, 36kD Phospho-tyrosine Adapter Protein, p36-38, pp36)

Description:
The adaptor molecule LAT (linker for activation of T cells) is a palmitoylated integral membrane protein of 36-38kD, localized to the glycolipid-enriched microdomains in the plasma membrane and plays an important role in linking engagement of the TCR to the biochemical events of T cell activation. Upon TCR engagement, LAT becomes phosphorylated on multiple tyrosine residues by ZAP-70 and Syk PTKs and binds Grb2, PLC-gamma1, the p85 subunit of PI3K, and other critical signaling molecules, thereby recruiting these molecules to the plasma membrane. It is required for TCR-mediated Ca2+ mobilization and optimal tyrosine phosphorylation of phospholipase C-gamma1, Vav and SLP-76. It is also required for Erk activation, CD69 up-regulation, and AP- and NFAT-mediated gene transcription. The binding of these proteins to LAT appears to be critical to TCR-mediated signaling. It is usually expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. LAT usually exists in two known isoforms.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 1:25-1:50
Optimal dilutions to be determined by the researcher.

Positive Control:
Tonsil

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	11E258	Supernatant

Size		Storage	Shipping	Source	Host
500ul		-20°C	Blue Ice	Human	Mouse

Concentration:

Not determined.

Immunogen:

Prokaryotic recombinant corresponding to region of 120aa with in the C-terminus of the LAT molecule. Cellular Localization: Membrane.

Purity:

Supernatant

Form

Supplied as a liquid, 0.05% sodium azide.

Specificity:

Recognizes human LAT.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Facchetti et al. J Pathol 154: 1037, 1999. 2. Fukazawa et al. The J Biol Chem 270: 20177, 1995. 3. Zhang et al. Cell 92: 83, 1998 4. Facchetti et al. American Journal of Pathology 154: 1037, 1999. 5. Zhang et al. International Immunology 11: 943, 1999.