Technical Data
Media
Lauryl Sulfate Broth (Powder)
L1400
Microbiological Grade
Storage RT    Shipping RT
Components shown as g/Liter
Formulation per Liter:
Pancreatic Digest of Casein20.0
Lactose5.0
Sodium Chloride5.0
Potassium Phosphate Dibasic2.75
Potassium Phosphate Monobasic2.75
Sodium Lauryl Sulfate0.1
Total per Liter35.51
500g makes 14Liters
Standard methods medium for growth of coliform bacteria in water and food.

Lauryl Sulfate Broth was formulated by Malimann and Darby (1) for detection of coliform bacteria in water. This is a selective medium because it contains sodium lauryl sulfate which inhibits Gram positive microorganisms. Lactose is added to detect rapid lactose fermentation by coliforms.

Quality Control Summary:
Expected cultural response after 18-24 hours at 35.0C..

Organism Result
Enterobacter aerogenes ATCC 1304 Growth, Gas +
8 Escherichia coli ATCC 25922 Growth, Gas +
Proteus mirabilis ATCC 12453 Growth, Gas -
Salmonella typhimurium ATCC 14028 Growth, Gas -
Staphylococcus aureus ATCC 25923 No Growth



Directions per Liter Dissolve 35.51 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized, adjusting pH as necessary. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage Store at 0-5C (store unopened media at 2-30C). Opened bottles should be capped tightly and kept in a dark, low humidity environment.

Appearance: White to off-white crystalline powder Solubility


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Mallmann, W. L., and C. W. Darby. 1941. Uses of a lauryl sulphate tryptose broth for the detection of coliform organsisms. Am J. Public Health. 31:127.