Technical Data
Leibovitz L-15ex Medium Modified w/Low Cupric Sulfate w/o Phenol Red
Molecular Biology Grade
Storage RT/4C    Shipping RT
Components shown as g/L
Calcium Chloride0.08
Cupric Sulfate0.0625
Magnesium Chloride0.11
Magnesium Sulfate0.11
Phenol RedAbsent
Potasssium Chloride0.23
Potassium Phosphate, Monobasic0.03
Sodium Chloride4.53
Sodium Phosphate, Dibasic0.11
Sodium Pyruvate0.31
L-15ex medium is a minimal medium based on Leibovitz L-15 basal cell culture medium. This medium, which was developed by Schirmer, et al. for studying the photocytotoxicity of polycyclic aromatic hydrodcarbons (PAHs) to the rainbow trout gill cell line, RTgill-W1 contains only salts, pyruvate and galactose. The simplicity of the medium favors expression of cellular responses to toxicants because protective molecules such as anti-oxidants are absent.

Directions per Liter: Dissolve 6.08g in 900ml of ddH2O with gentle stirring stirring until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi). Alternatively, instead of autoclaving, the liquid can be sterile-filtered using a 0.22um membrane. Supplements can be introduced aseptically to sterile medium.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: White to off-white, homogeneous, free flowing powder

Solubility: Colorless, clear, complete. Precipitate will form.

pH: As reported

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Schirmer K, et al. 1997. Methodology for demonstrating and measuring the photocytotoxicity of fluoranthene to fish cells in culture. Toxicology In Vitro. 11:107-119.