Technical Data
L1566-02A
LIME1 (Lck-interacting Transmembrane Adapter 1, Lck-interacting Membrane Protein, Lck-interacting Molecule, LIME, LP8067)
Description:
LIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2aLIME-10Affinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa281-296 from human LIME1 (KLH).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 15mM sodium azide, pH 7.4.
Specificity:
Recognizes human LIME1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.