		Technical Data
L1566-06A	LIME (Lck-interacting Transmembrane Adapter 1, Lck-interacting Molecule, Lime1)

Description:
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml
ELISA: >1:128,000
Optimal dilutions to be determined by the researcher.

Positive Control:
Mouse heart

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Mouse	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to ENVYESIKEMGL, from the C-terminus of the protein sequence according to NP_076173 (mouse).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.

Specificity:

Recognizes mouse LIME. Species sequence homology: rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Hur EM, Son M, Lee OH, Choi YB, Park C, Lee H, Yun Y. LIME, a novel transmembrane adaptor protein, associates with p56lck and mediates T cell activation. J Exp Med. 2003 Nov 17;198(10):1463-73. Epub 2003 Nov 10. PMID: 14610044. 2. Brdicková J. Exp.Med.198 (10); 1453-1462.