Technical Data
L1566-06A
LIME (Lck-interacting Transmembrane Adapter 1, Lck-interacting Molecule, Lime1)
Description:
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml
ELISA: >1:128,000
Optimal dilutions to be determined by the researcher.

Positive Control:
Mouse heart

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouseGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to ENVYESIKEMGL, from the C-terminus of the protein sequence according to NP_076173 (mouse).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity:
Recognizes mouse LIME. Species sequence homology: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hur EM, Son M, Lee OH, Choi YB, Park C, Lee H, Yun Y. LIME, a novel transmembrane adaptor protein, associates with p56lck and mediates T cell activation. J Exp Med. 2003 Nov 17;198(10):1463-73. Epub 2003 Nov 10. PMID: 14610044. 2. Brdicková J. Exp.Med.198 (10); 1453-1462.