Technical Data
L1662-86
Lectin, Mannose-binding 1 (LMAN1, Endoplasmic Reticulum-Golgi Intermediate Compartment Protein 53, Intracellular Mannose Specific Lectin)
Description:
The ER-Golgi intermediate compartment (ERGIC) marker ERGIC-53 is a mannose-specific membrane lectin operating as a cargo receptor for the transport of glycoproteins from the ER to the ERGIC. ERGIC-53 can also be used for studying aspects of protein trafficking in the secretory pathway.

Applications:
Suitable for use in ELISA, Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:250
Immunoprecipitation: 2ul
Immunohistochemistry: Frozen sections
Immunocytochemistry: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile PBS, 0.1% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG16D523Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Human ERGIC-53 from Caco-2 cell golgi membrane fraction.
Purity:
Purified by HYDRA chromatography and gel filtraton (FPLC).
Form
Supplied as a lyophilized powder with no additives. Reconstitute with 100ul sterile PBS, 0.1% sodium azide.
Specificity:
Recognizes human ERGIC-53. Species Crossreactivity: porcine and monkey
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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