Technical Data
Lectin, Mannose-binding 1 (LMAN1, Endoplasmic Reticulum-Golgi Intermediate Compartment Protein 53, Intracellular Mannose Specific Lectin)
The ER-Golgi intermediate compartment (ERGIC) marker ERGIC-53 is a mannose-specific membrane lectin operating as a cargo receptor for the transport of glycoproteins from the ER to the ERGIC. ERGIC-53 can also be used for studying aspects of protein trafficking in the secretory pathway.

Suitable for use in ELISA, Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:250
Immunoprecipitation: 2ul
Immunohistochemistry: Frozen sections
Immunocytochemistry: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile PBS, 0.1% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG16D523Affinity Purified
100ug-20CBlue IceHumanMouse
~1mg/ml (after reconstitution)
Human ERGIC-53 from Caco-2 cell golgi membrane fraction.
Purified by HYDRA chromatography and gel filtraton (FPLC).
Supplied as a lyophilized powder with no additives. Reconstitute with 100ul sterile PBS, 0.09% sodium azide.
Recognizes human ERGIC-53. Species Crossreactivity: porcine and monkey
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Schweizer, A., et al. (1988) J. Cell Biol. 107:1643. 2. Schweizer, A., et al. (1991) J. Cell Biol. 113:45. 3. Kappeler, F., et al., (1994) J. Biol. Chem. 269:6279. 4. Itin, C., et al., (1995) J. Cell Biol. 131:57. 5. Itin, C., et al., (1996) Mol. Biol. Cell 7:483. 6. Kappeler, F., et al. (1997) J. Biol. Chem. 272:31,801. 7. Halaban, R., et al., (2002) J. Biol. Chem. 277:14,821. 8. Appenzeller-Herzog, C., et al., (2004) J. Biol. Chem. 279:12,943. 9. Breuza, L., et al., (2004) J. Biol. Chem. 279:47,242. 10. Hauri, H.P., et al., (2000) J. Cell Sci. 113:587.