Technical Data
L1669-03A
Leptin (Ob, Obese Protein, Obesity Factor)
Description:
Secreted leptin associates with proteins within the bloodstream and is transported to the brain, where it inhibits or up-regulates the activity of neuropeptides such as neuropeptide Y (NPY) and alpha-melanocyte-stimulating hormone respectively. Leptin is a recognised marker of obesity, with a correlation between circulating levels of leptin and body fat mass, and defects in leptin production result in severe obesity. Regulators of leptin expression include insulin, cortisol, cAMP and thiazolidinediones.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-0.2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, ddH2O, 0.09% sodium azide. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Recombinant human leptin.
Purity:
Purified
Form
Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with 100ul sterile 40-50% glycerol, ddH2O, 0.09% sodium azide.
Specificity:
Recognizes human leptin, at 16kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Paracchini, V. et al. (2005) Genetics of leptin and obesity: a HuGE review. Am. J. Epidemiol. 162: 101-114.