Technical Data
L1669-03B
Leptin (Ob, Obese Protein, Obesity Factor) (Biotin)
Description:
Leptin, a 16KD major adipose-derived hormone and member of the leptin family, which plays a key role in appetite control and the regulation of energy expenditure.
Secreted leptin associates with proteins within the bloodstream and is transported to the brain, where it inhibits or up-regulates the activity of neuropeptides such as neuropeptide Y (NPY) and alpha-melanocyte-stimulating hormone respectively. Leptin is a recognised marker of obesity, with a correlation between circulating levels of leptin and body fat mass, and defects in leptin production result in severe obesity. Regulators of leptin expression include insulin, cortisol, cAMP and thiazolidinediones.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.25-1ug/ml
Western Blot: 0.1- 0.2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute by adding sterile 40-50% glycerol, ddH2O. Aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Recombinant human leptin.
Purity:
Purified by affinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.2.Labeled with Biotin. Reconstitute with 0.5ml sterile 40-50% glycerol, PBS, 0.1% BSA.
Specificity:
Recognizes human leptin, a 16KD major adipose-derived hormone and member of the leptin family, which plays a key role in appetite control and the regulation of energy expenditure.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Paracchini, V. et al. (2005) Genetics of leptin and obesity: a HuGE review. Am. J. Epidemiol. 162: 101-114.