Technical Data
L1669-06
Leptin (Ob, Obese Protein, Obesity Factor)
Description:
Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilution:
ELISA (Direct and Indirect): 1:5000-1:10,000
Western Blot: 1:5000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk. Incubate for 1 hour at RT.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabPurified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
Recombinant mouse leptin.
Purity:
Purified.
Form
Supplied as a liquid in PBS, pH 7.4. No preservative added.
Specificity:
Recognizes human (weak), mouse and rat (weak) leptin. Detects a band of ~16kDa by Western blot.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.